Immediately after graduation, Vlatka Pavlović began her internship at the University Clinical Hospital Center "Sestre Milosrdnice" Neurosurgery Clinic, where she worked as a nurse in the post-intensive medical care unit until 2005.

In the same year, she was employed as a nurse at the Klaićeva Clinic for Children's Diseases, in the Intensive Care Unit, where she worked until 2006.

In 2006, she started working in Antonija Čavala Miljan's general practice as a nurse until 2020, and at the same time enrolled in a study-as-you-work course for nurses at the University of Applied Health Sciences in Zagreb, where she obtained the title of Bachelor of Nursing in 2022.

From 2020 to 2023, she worked at Dubrava University Hospital at the Department of Nephrology and Dialysis, and during the Covid-19 epidemic, she worked at the respiratory center of Dubrava University Hospital.